کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7233481 | 1470974 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Microfluidic bead-based sensing platform for monitoring kinase activity
ترجمه فارسی عنوان
پلت فرم سنجش بر اساس میکرو فلوئیدیک برای نظارت بر فعالیت کیناز
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Protein kinases control cellular functions by regulating protein phosphorylation. Monitoring protein kinase activity is essential for medical diagnosis and drug screening. Here, we present a novel microfluidic device for performing simple and versatile protein kinase assays, which utilizes a microbead-based chemosensor. An automatic mix-and-measure technique was achieved using integrated pneumatic valves. After mixing each reagent for the kinase assay, the mixture was transferred to the sensing chamber. Then, phosphorylated and fluorescence-labeled peptides were captured and detected by the chemosensor. A fluorescence signal was observed depending on the presence of the kinase. Furthermore, activities of various kinases in the cell lysate and the inhibitory effect of specific chemicals on the kinases were monitored. These results indicate that chemosensor-based microfluidic chips can be developed as a versatile kinase assay system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 57, 15 July 2014, Pages 1-9
Journal: Biosensors and Bioelectronics - Volume 57, 15 July 2014, Pages 1-9
نویسندگان
Seung Hwan Lee, Hyun-Woo Rhee, Danny van Noort, Hong Jai Lee, Hee Ho Park, Ik-Soo Shin, Jong-In Hong, Tai Hyun Park,