کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7233554 | 1470972 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Graphene functionalized porous Au-paper based electrochemiluminescence device for detection of DNA using luminescent silver nanoparticles coated calcium carbonate/carboxymethyl chitosan hybrid microspheres as labels
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Graphene functionalized porous Au-paper based electrochemiluminescence device for detection of DNA using luminescent silver nanoparticles coated calcium carbonate/carboxymethyl chitosan hybrid microspheres as labels Graphene functionalized porous Au-paper based electrochemiluminescence device for detection of DNA using luminescent silver nanoparticles coated calcium carbonate/carboxymethyl chitosan hybrid microspheres as labels](/preview/png/7233554.png)
چکیده انگلیسی
In the paper, a simple and sensitive electrochemiluminescence (ECL) DNA sensor based on graphene-modified porous Au-paper working electrode (GR/Au-PWE) and calcium carbonate/carboxymethyl chitosan (CaCO3/CMC) hybrid microspheres @ luminescent silver nanoparticles (AgNPs) composites was developed. The GR/Au-PWE with excellent conductivity was successfully prepared for the immobilization of capture probe. The CaCO3/CMC hybrid microspheres were prepared by the precipitation of calcium carbonate in an aqueous solution containing CMC. The AgNPs was synthesized by thermal reduction of silver ions in glycine matrix, taking advantage of the solid-state matrix to control the nucleation and migration of reduced silver atoms. The CaCO3/CMC@AgNPs composites exhibited 3.6 times higher ECL intensity than the pure AgNPs-labeled reporter DNA. Taking advantage of dual-amplification effects, the paper-based DNA sensor could detect the target DNA quantitatively, in the range of 4.0Ã10â17-5.0Ã10â11Â M, with a limit of detection as low as 8.5Ã10â18Â M, and perform excellent selectivity. The simple, low-cost, sensitive device could be easily applied for point-of-care testing, public health and environmental monitoring in remote regions, developing or developed countries.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 59, 15 September 2014, Pages 307-313
Journal: Biosensors and Bioelectronics - Volume 59, 15 September 2014, Pages 307-313
نویسندگان
Meng Li, Yanhu Wang, Yan Zhang, Jinghua Yu, Shenguang Ge, Mei Yan,