کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7233569 | 1470972 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Copper-doped titanium dioxide nanoparticles as dual-functional labels for fabrication of electrochemical immunosensors
ترجمه فارسی عنوان
نانوذرات دی اکسید تیتانیوم با دوز مضاعف به عنوان برچسبهای دوگانه برای تولید ایمونوسنسورهای الکتروشیمیایی
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Constructions of versatile electroactive labels are key issues in the development of electrochemical immunosensors. In this study, copper-doped titanium dioxide nanoparticle (Cu@TiO2) was synthesized and used as labels for fabrication of sandwich-type electrochemical immunosensors on glassy carbon electrode (GCE). Due to the presence of copper ions, Cu@TiO2 shows a strong response current when coupled to an electrode. The prepared nanocomposite also shows high electrocatalytic activity towards reduction of hydrogen peroxide (H2O2). The dual functionality of Cu@TiO2 enables the fabrication of immunosensor using different detection modes, that is, square wave voltammetry (SWV) or chronoamperometry (CA). While Cu@TiO2 was used as labels of secondary antibodies (Ab2), carboxyl functionalized graphene oxide (CFGO) was used as electrode materials to immobilize primary antibodies (Ab1). Using human immunoglobulin G (IgG) as a model analyte, the immunosensor shows high sensitivity, acceptable stability and good reproducibility for both detection modes. Under optimal conditions, a linear range from 0.1Â pg/mL to 100Â ng/mL with a detection limit of 0.052Â pg/mL was obtained for SWV analysis. For CA analysis, a wider linear range from 0.01Â pg/mL to 100Â ng/mL and a lower detection limit of 0.0043Â pg/mL were obtained. The proposed metal ion-based enzyme-free and noble metal-free immunosensor may have promising applications in clinical diagnoses and many other fields.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 59, 15 September 2014, Pages 335-341
Journal: Biosensors and Bioelectronics - Volume 59, 15 September 2014, Pages 335-341
نویسندگان
Sen Zhang, Hongmin Ma, Liangguo Yan, Wei Cao, Tao Yan, Qin Wei, Bin Du,