کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7233684 | 1470977 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Microconductometric immunosensor for label-free and sensitive detection of Gram-negative bacteria
ترجمه فارسی عنوان
ایمنوسنسور میکروکانودوتومتری برای تشخیص برچسب و حساس باکتریهای گرم منفی
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کلمات کلیدی
ایمنی خون، باکتری های گرم منفی، مغناطیس ایمنی هدایت متریک، تشخیص رایگان برچسب
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Blood safety is a global health goal. In developed countries, bacterial contamination of platelet concentrates is the highest infectious risk in transfusion despite the current preventive strategies. We aimed to develop a conductometric biosensor for the generic, rapid and sensitive detection of Gram-negative bacteria. Our strategy is based on immunosensors: addressable magnetic nanoparticles coupled with anti-LPS antibodies were used for the generic capture of Gram-negative bacteria. Bacterial capture was characterized by impedancemetric and microscopic measurements. The results obtained with conductometric measurements allowed real-time, sensitive detection of Escherichia coli or Serratia marcescens cultures from 1 to 103Â CFUÂ mLâ1. The ability of the immunosensor to detect Gram negative bacteria was also tested on clinically relevant strains. The conductometric immunosensor allowed the direct detection of 10-103Â CFUÂ mLâ1 of Pseudomonas aeruginosa and Acinetobacter baumannii strains that were undetectable using standard immunoblot methods. Results showed that the conductometric response was not inhibited in 1% serum.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 54, 15 April 2014, Pages 378-384
Journal: Biosensors and Bioelectronics - Volume 54, 15 April 2014, Pages 378-384
نویسندگان
Sarra El Ichi, Fanny Leon, Ludivine Vossier, Helene Marchandin, Abdelhamid Errachid, Joliette Coste, Nicole Jaffrezic-Renault, Chantal Fournier-Wirth,