کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7233702 | 1470977 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Microalgae dual-head biosensors for selective detection of herbicides with fiber-optic luminescent O2 transduction
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
The microalgal species Dictyosphaerium chlorelloides (D. c.) was immobilized into porous silicone films and their photosynthetic activity was monitored with an integrated robust luminescent O2 sensor. The biosensor specificity towards a particular pesticide has been achieved by manufacturing a fiber-optic dual-head device containing both analyte-sensitive and analyte-resistant D. c. strains. The latter are not genetically modified microalgae, but a product of modified Luria-Delbrück fluctuation analysis followed by ratchet selection cycles. In this way the target herbicide decreases the O2 production of the analyte-sensitive immobilized strain without affecting the analyte-resistant population response; any other pollutant will lower the O2 production of both strains. The effect of the sample flow-rate, exposure time to the herbicide, biomass loading, biosensor film thickness, intensity of the actinic light, illumination cycle, and temperature on the biosensor response has been evaluated using waterborne simazine as test bench. The biosensing device is able to provide in situ measurements of the herbicide concentration every 180 min. The biosensor limit of detection for this herbicide was 12 μg Lâ1, with a working range of 50-800 μg Lâ1. The biosensor specificity to simazine has been assessed by comparing its response to that of isoproturon.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 54, 15 April 2014, Pages 484-491
Journal: Biosensors and Bioelectronics - Volume 54, 15 April 2014, Pages 484-491
نویسندگان
David Haigh-Flórez, Cristina de la Hera, Eduardo Costas, Guillermo Orellana,