کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7233970 | 1470981 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A carbon nanotube metal semiconductor field effect transistor-based biosensor for detection of amyloid-beta in human serum
ترجمه فارسی عنوان
بیوسنسور مبتنی بر ترانزیستور میدان نیمه هادی نانولوله کربنی برای تشخیص آمیلوئید بتا در سرم انسان
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
We have developed a carbon nanotube (CNT) film-based biosensor with a metal semiconductor field effect transistor structure (MESFET). A gold top gate was deposited on the middle of the CNT channel and probe antibodies were immobilized on the gold top gate with an antibody-binding protein, protein G or Escherichia coli outer membrane (OM) with autodisplayed Z-domains of protein A. These CNT-MESFET biosensors exhibited a higher sensitivity than the CNT-FET biosensor with probe antibodies immobilized using a chemical linker, since the orientation of immobilized antibodies was controlled by the antibody-binding proteins. In addition, nonspecific binding was effectively inhibited by E. coli OM. Using the CNT-MESFET biosensors with E. coli OM containing Z domain, we detected amyloid-β (Aβ) in human serum, one of the biomarkers for early diagnosis of Alzheimer's disease. Aβ at the level of 1 pg/mL in human serum could be measured in real-time and without labeling, which was lower than a limit of detection for plasma Aβ using an enzyme-linked immune sorbent assay. These results suggested that our CNT-MESFET biosensors might be applicable for an early diagnosis of Alzheimer's disease.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 50, 15 December 2013, Pages 345-350
Journal: Biosensors and Bioelectronics - Volume 50, 15 December 2013, Pages 345-350
نویسندگان
Jeseung Oh, Gu Yoo, Young Wook Chang, Hyung Joon Kim, Joachim Jose, Eosu Kim, Jae-Chul Pyun, Kyung-Hwa Yoo,