کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7233984 1470981 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electrochemical immunosensor for simultaneous detection of multiplex cancer biomarkers based on graphene nanocomposites
ترجمه فارسی عنوان
ایمونوسنسور الکتروشیمیایی برای تشخیص همزمان بیومارکرهای چندتایی سرطان بر اساس نانوکامپوزیتهای گرافن
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
In this work, a sandwich-format electrochemical immunosensor for simultaneous determination of carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) was fabricated using biofunctional carboxyl graphene nanosheets (CGS) as immunosensing probes, which were fabricated by means of immobilization of toluidine blue (TB) and labeled anti-CEA (Ab2,1), Prussian blue (PB) and anti-AFP (Ab2,2) successively on CGS. The capture anti-CEA (Ab1,1) and anti-AFP (Ab1,2) were immobilized onto the chitosan-Au nanoparticles (CHIT-AuNPs) modified electrode through 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxy succinimide (EDC/NHS). Experimental results revealed that this sandwich-type immunoassay enabled simultaneous detection of CEA and AFP with linear range of 0.5-60 ng mL−1 for both analytes. The detection limit was 0.1 ng mL−1 for CEA and 0.05 ng mL−1 for AFP (S/N=3). The assay results of serum samples with the proposed method were in a good agreement with the reference values from the standard ELISA method. And the negligible cross-reactivity between the two analytes allows it to possess potential promise in clinical diagnosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 50, 15 December 2013, Pages 356-361
نویسندگان
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