کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7234065 | 1470982 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Stable label-free fluorescent sensing of biothiols based on ThT direct inducing conformation-specific G-quadruplex
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Stable label-free fluorescent sensing of biothiols based on ThT direct inducing conformation-specific G-quadruplex Stable label-free fluorescent sensing of biothiols based on ThT direct inducing conformation-specific G-quadruplex](/preview/png/7234065.png)
چکیده انگلیسی
In this work, a new, label-free, turn-on fluorescent sensor for biothiols detection based on ThT direct inducing conformation-specific G-quadruplex is developed. The sensing approach is based on a conformational switch of oligonucleotide controlled by Hg2+ and a commercially available water-soluble fluorescent dye, Thioflavin T (ThT). A noticeable fluorescence light-up in ThT on binding to the G-quadruplex grants the sensor excellent sensitivity. The specific quadruplex conformation induced directly by ThT and pronounced structural selectivity of ThT for G-quadruplexes could generate more stable luminescence and make sure high specificity in complex biological samples. The present assay allows for the selective determination of cysteine and glutathione in the range of 2.0Ã10â8-2.5Ã10â6Â M and 3.0Ã10â8-2.0Ã10â6Â M with a detection limit of 8.4Â nM and 13.9Â nM respectively. The diagnostic capability and potential in practical applications of this method have been demonstrated by detecting biothiols in human blood serum.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 49, 15 November 2013, Pages 420-425
Journal: Biosensors and Bioelectronics - Volume 49, 15 November 2013, Pages 420-425
نویسندگان
Li-li Tong, Lu Li, Zhenzhen Chen, Qian Wang, Bo Tang,