Direct electron transfer with enzymes on nanofiliform titanium oxide films with electron-transport ability

► Nanofiliform titanium oxide films (nTOFs)/Ti are fabricated by a simple wet-corrosion-process. ► Direct electron transfer (DET) with enzyme on nTOFs can be achieved. ► Size of aggregated nanofiliform below 100 nm is contributed to the DET. ► HRP/nTOFs/Ti-electrodes can detect hydrogen peroxide based on DET up to 100 μM.