کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7234478 | 1470990 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
CRP detection from serum for chip-based point-of-care testing system
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Most of point-of-care testing (POCT) to improve facilitates in diagnosis, treatment, and monitoring of patients. POCT technique has still remained a quantitatively and accurately detective effect. In this article, we demonstrated that real human C-reactive protein (CRP) in serum was detected for a chip-based point-of-care testing application based on a nanogap-embedded field effect transistor (FET), and the results were compared with those obtained via the enzyme-linked immunosorbent assay (ELISA) method. The limit of detection (LOD), determined from the standard curve, was 0.1Â ng/ml, which is comparable to that of commercialized ELISAs. We evaluated that an improved detection range (0.1Â ng/ml to 100Â ng/ml) was achieved by comparing with commercialized ELISA. Control experiments to determine selectivity and to discern false-positive/false-negative rates were also performed. This report is the first description of the detection of CRP in human serum using a silicon-based biosensor.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 41, 15 March 2013, Pages 322-327
Journal: Biosensors and Bioelectronics - Volume 41, 15 March 2013, Pages 322-327
نویسندگان
Chang-Hoon Kim, Jae-Hyuk Ahn, Jee-Yeon Kim, Ji-Min Choi, Kyung-Choon Lim, Tae Jung Park, Nam Su Heo, Hee Gu Lee, Jong-Wan Kim, Yang-Kyu Choi,