A novel and rapid assay for HIV-1 protease detection using magnetic bead mediation

► Establishment of simple and inexpensive sensitive monolayer for HIV-1 protease detection. ► The sensitivity and the response time of the biosensor improved using nano-magnetic beads. ► Specificity of HIV-1 protease detection confirmed using impedance and Raman spectroscopy techniques. ► The sensing platform employed for AIDS drugs screening without using washing liquids.