Highly sensitive and selective chemiluminescent imaging for DNA detection by ligation-mediated rolling circle amplified synthesis of DNAzyme

► This work constructs a highly sensitive chemiluminescent imaging method. ► A novel signal amplification strategy to enhance the sensitivity is proposed. ► Ligation-mediated rolling circle amplification of DNAzyme is introduced to CL imaging. ► This biosensor can detect target DNA down to sub-femtomolar level.