Three-dimensional paper-based electrochemiluminescence device for simultaneous detection of Pb2+ and Hg2+ based on potential-control technique

► A microfluidic paper-based analytical device was proposed by electrochemiluminescence method. ► Pb2+ and Hg2+ were detected in one paper working zone based on potential-control technique. ► The Si@CNCs and Ru@AuNPs with different operational potentials were used as ECL labels. ► The device was used for simultaneous detection of Pb2+and Hg2+ in lake water and human serum samples, respectively.