کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7234787 | 1470991 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Using a cell-based gas biosensor for investigation of adverse effects of acetone vapors in vitro
ترجمه فارسی عنوان
با استفاده از یک بیوسنسور گاز مبتنی بر سلول برای بررسی اثرات نامطلوب بخارات استون در آزمایشگاهی
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
In this study, a cell-based gas biosensor is presented used for the detection and investigation of gaseous organic compounds in air. The response of living human nasal cells (RPMI 2650) and human lung cells (A549) towards the direct exposure of gaseous substances for 10Â min is monitored with a multi-parametric sensor system. Changes in the cellular impedance, oxygen consumption rate and acidification rate can be recorded after the exposure and represent the cytotoxicity of the present gas. The sensor is able to notify the presence of acetone in aqueous solution (2%) but in notably lower concentrations in the gas phase (100-333Â ppm) within 30-60Â min after the end of the gas exposure. Cell viability is not affected by a sequential exposure to humidified synthetic air (60%Â r.h.) with a flow rate of 300Â ml/min and therefore offers the possibility for a continuous air monitoring. In addition, exposure to synthetic air has no influence on the signals of consecutive acetone exposure. The system might be used in the future for the monitoring of ambient air in work spaces.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 40, Issue 1, 15 February 2013, Pages 393-400
Journal: Biosensors and Bioelectronics - Volume 40, Issue 1, 15 February 2013, Pages 393-400
نویسندگان
Ulrich Bohrn, Evamaria Stütz, Maximilian Fleischer, Michael J. Schöning, Patrick Wagner,