Bioengineered olfactory sensory neuron-based biosensor for specific odorant detection

► A bioengineered olfactory sensory neurons (OSNs)-based biosensor was developed. ► This biosensor could specifically respond to ligand of expressed ORs in a dose-dependent way. ► The extracellular firings of bioengineered OSNs were analyzed in frequency and time domains. ► The temporal firing response modes were different at low/high concentration of odorant.