کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7234947 | 1471014 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Purification and biochemical characterization of an Aspergillus niger phytase produced by solid-state fermentation using triticale residues as substrate
ترجمه فارسی عنوان
تعیین و تعیین خصوصیات بیوشیمیایی فتاز آسپرژیلوس نایگر توسط بقایای حالت جامد با استفاده از ترکیبات تریتیکاله به عنوان سوبسترا
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی انرژی
انرژی های تجدید پذیر، توسعه پایدار و محیط زیست
چکیده انگلیسی
In this study, an extracellular phytase produced by Aspergillus niger 7A-1, was biochemically characterized for possible industrial application. The enzyme was purified from a crude extract obtained by solid-state fermentation (SSF) of triticale waste. The extract was obtained by microfiltration, ultrafiltration (300, 100 and 30â¯kDa) and DEAE-Sepharose column chromatography. The molecular weight of the purified enzyme was estimated to be 89â¯kDa by SDS-PAGE. The purified enzyme was most active at pH 5.3 and 56â¯Â°C, and retained 50% activity over a wide pH range of 4 to 7. The enzymatic thermostability assay showed that the enzyme retained more than 70% activity at 80â¯Â°C for 60â¯s, 40% activity for 120â¯s and 9% after 300â¯s. The phytase showed broad substrate specificity, a Km value of 220â¯Î¼M and Vmax of 25â¯Î¼M/min. The purified phytase retained 50% of its activity with phosphorylated compounds such as phenyl phosphate, 1-Naphthyl phosphate, 2-Naphthyl phosphate, p-Nitrophenyl phosphate and Glycerol-2-phosphate. The inhibition of phytase activity by metal ions was observed to be drastically inhibited (50%) by Ca++ and was slightly inhibited (10%) by Ni++, K+, and Na+, at 10 and 20â¯mM concentrations. A positive effect was obtained with Mg++, Mn++, Cu++, Cd++ and Ba++ at 25 and 35% with stimulatory effect on the phytase activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biotechnology Reports - Volume 17, March 2018, Pages 49-54
Journal: Biotechnology Reports - Volume 17, March 2018, Pages 49-54
نویسندگان
Alberto A. Neira-Vielma, Cristóbal N. Aguilar, Anna Ilyina, Juan C. Contreras-Esquivel, MarÃa das Graça Carneiro-da-Cunha, Georgina Michelena-Álvarez, José L. MartÃnez-Hernández,