Efficient gene editing via non-viral delivery of CRISPR-Cas9 system using polymeric and hybrid microcarriers

CRISPR-Cas9 is a revolutionary genome-editing technology with huge potential for research, but also clinical applications. Microcapsules combine high loading capacity with easy functionalization, e.g., to allow tissue targeting. Using an indicator cell line we show here that polymeric and hybrid silica-coated capsules mediate efficient, non-toxic and transient delivery of CRISPR-Cas9 system components. Our data suggest that microcapsules represent promising vectors for application of genome-editing tools.244