A self-assembled monolayer on gold nanoparticles modified electrode for simultaneous determination of isoproterenol and uric acid

• DPB self-assembled monolayer on gold nanoparticle modified GCE was prepared.
• The prepared electrode was characterized by SEM, EIS and CV methods.
• The modified electrode can catalyze the oxidation of isoprenaline.
• This electrode was used as an electrochemical sensor for the detection of isoprenaline and uric acid.
• The detection limit of isoprenaline by the sensor was 82 nM.

A novel layer-by-layer assembly of gold nanoparticles (AuNPs) and 2-(2,3-dihydroxy phenyl) benzothiazole (DPB) was successfully applied at a glassy carbon electrode (GCE/AuNPs/DPB). At first, AuNPs were deposited on the GCE by chemical and electrochemical methods and then DPB is self-assembled on the Au nanoparticles. This new modified electrode system was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). GCE/AuNPs/DPB was used for simultaneous determination of isoproterenol (IP) and uric acid (UA). It was observed that an electrode with electrochemical deposited of Au nanoparticles has higher electrocatalytic activity for the oxidation of IP in comparison of the electrode with chemical deposited of Au nanoparticles. Also results show the DPB has a key role in electrocatalysis of IP and in simultaneous determination of procedure because the DPB can catalysis the oxidation of IP via EC′ mechanism. Differential pulse voltammetry (DPV) exhibited a linear dynamic range over the concentration range of 0.1–900.0 μM and a detection limit (3 s) of 82 nM for IP in the optimum conditions. Finally, DPV was used for simultaneous determination of IP and UA and their detection in real samples.

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