A paper-based amperometric glucose biosensor developed with Prussian Blue-modified screen-printed electrodes

• Glucose oxidase (GOx) was physically adsorbed on paper without use of chemicals.
• This biosensor configuration was cost-effective and showed good stability.
• Simple platform for applications that require small sample volumes.

This paper describes a simple inexpensive paper-based amperometric glucose biosensor developed based on Prussian Blue (PB)-modified screen-printed carbon electrodes (SPCEs). The use of cellulose paper proved to be a simple, “ideal” and green biocompatible immobilization matrix for glucose oxidase (GOx) as it was successfully embedded within the fibre matrix of paper via physical adsorption. The glucose biosensor allowed a small amount (0.5 μL) of sample solution for glucose analysis. The biosensor had a linear calibration range between 0.25 mM and 2.00 (R2 = 0.987) and a detection limit of 0.01 mM glucose (S/N = 3). Interference study of selected potential interfering compounds on the biosensor response was investigated. Its analytical performance was demonstrated in the analysis of selected commercial glucose beverages. Despite the simplicity of the immobilization method, the biosensor retained ca. 72% of its activity after a storage period of 45 days.

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