کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
745502 1462118 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
DNA hybridization mechanism in an interfacial environment: What hides beneath first order k (s−1) kinetic constant?
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
DNA hybridization mechanism in an interfacial environment: What hides beneath first order k (s−1) kinetic constant?
چکیده انگلیسی

The scientific question addressed in this work is: what hides beneath first order kinetic constant k (s−1) measured for hybridization of a DNA target on a biosensor surface. Kinetics hybridization curves were established with a 27 MHz quartz microbalance (9 MHz, third harmonic) biosensor, constituted of a 20-base probe monolayer deposited on a gold covered quartz surface. Kinetics analysis, by a known two-step adsorption–hybridization mechanism, is well appropriate to fit properly hybridization kinetics curves, for complementary 20-base to 40-base targets over two concentration decades. It was found that the K1 (M−1) adsorption constant, relevant to the first step, concerns an equilibrium between non hybridized targets and hybridized pre-complex and increases with DNA target length. It was established that k2 (s−1), relevant to irreversible formation of a stable duplex, varies in an opposite way to K1 with DNA target length.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volumes 171–172, August–September 2012, Pages 522–527
نویسندگان
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