کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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745778 | 894430 | 2011 | 5 صفحه PDF | دانلود رایگان |

A simple and novel potentiometric biosensor for urea detection was prepared by employing an electrosynthesized polymer with buffering capability. It was obtained by deposition of a weighed amount of urease (Ur) at a glassy carbon (GC) electrode followed by immobilization by an electrosynthesized poly-o-phenylenediamine (PPD) film. An unconventional “upside-down” (UD) geometry was employed for the electrochemical cell. The response of GC/Ur/PPD sensor is linear with urea concentration in the range 10 μM to 1 mM (15 mV/mM, R2 = 0.9999) due to buffering capability of PPD film, which represents a novel role of electrosynthesized polymers in their application to biosensors. At higher concentrations, the more common Nernstian response (28 mV/decade, R2 = 0.9987) is observed. The sensor exhibits a sufficient sensitivity for practical determinations, rapid response and long term stability.
Journal: Sensors and Actuators B: Chemical - Volume 157, Issue 1, 20 September 2011, Pages 211–215