کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
751780 895261 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An electrochemical tyrosinase-immobilized biosensor for albumin—toward a potential total protein measurement
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
An electrochemical tyrosinase-immobilized biosensor for albumin—toward a potential total protein measurement
چکیده انگلیسی

A simple biosensor, based on a tyrosinase-immobilized electrode, was developed for a rapid and quantitative measurement of bovine serum albumin (BSA) and human serum albumin (HSA). Tyrosinase, immobilized on a screen-printed carbon electrode, catalyzed the oxidation of tyrosine residues in the albumin to the corresponding quinone, which was further reduced electrochemically by the sensing electrode under an appropriate condition. The concentration of the protein, therefore, could be quantified by measuring the reduction current. The operational parameters that affect the performance of this biosensor, e.g., the working potential, the pH value, and the temperature, were assessed and optimized. The stability, the lifetime, and the reproducibility of this biosensor were also evaluated. This biosensor demonstrated that it was not only a highly sensitive assay for albumin but will be a potential tool for the measurement of total protein in human serum.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 114, Issue 1, 30 March 2006, Pages 357–363
نویسندگان
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