کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7557965 | 1491357 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differentiating between monozygotic twins through next-generation mitochondrial genome sequencing
ترجمه فارسی عنوان
تمایز بین دوقلوهای مونوزیگوت با توالی بندی ژنوم میتوکندری نسل بعدی
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کلمات کلیدی
Mitochondrial genome (mtGenome)revised Cambridge Reference SequencerCRSSTRIGVPHpSTSMitochondrial DNA - DNA میتوکندریاMassively parallel sequencing - توالی انعطاف پذیری موازیShort tandem repeat - تکرار کوتاه مدتMonozygotic twins - دوقلوهای مونوزیگوتmtDNA - دیانای میتوکندریاییIntegrative Genomics Viewer - مجتمع ژنومیک بینندهMonozygotic - مونوزیگوتMPs - نمایندگان مجلسpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازSingle nucleotide polymorphism - پلیمورفیسم تک نوکلئوتیدیSNP - چندریختی تک-نوکلئوتیدForensic genetics - ژنتیک قانونیMitochondrial genome - ژنوم میتوکندریابی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Monozygotic (MZ) twins, considered to be genetically identical, cannot be distinguished from one another by standard forensic DNA testing. A recent study employed whole genome sequencing to identify extremely rare mutations and reported that mutation analysis could be used to differentiate between MZ twins. Compared with nuclear DNA, mitochondrial DNA (mtDNA) has higher mutation rates; therefore, minor differences theoretically exist in MZ twins' mitochondrial genome (mtGenome). However, conventional Sanger-type sequencing (STS) is neither amenable to, nor feasible for, the detection of low-level sequence variants. The recent introduction of massively parallel sequencing (MPS) has the capability to sequence many targeted regions of multiple samples simultaneously with desirable depth of coverage. Thus, the aim of this study was to assess whether full mtGenome sequencing analysis can be used to differentiate between MZ twins. Ten sets of MZ twins provided blood samples that underwent extraction, quantification, mtDNA enrichment, library preparation, and ultra-deep sequencing. Point heteroplasmies were observed in eight sets of MZ twins, and a single nucleotide variant (nt15301) was detected in five sets of MZ twins. Thus, this study demonstrates that ultra-deep mtGenome sequencing could be used to differentiate between MZ twins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 490, 1 December 2015, Pages 1-6
Journal: Analytical Biochemistry - Volume 490, 1 December 2015, Pages 1-6
نویسندگان
Zheng Wang, Ruxin Zhu, Suhua Zhang, Yingnan Bian, Daru Lu, Chengtao Li,