کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7584368 | 1492023 | 2018 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rapid detection of β-conglutin with a novel lateral flow aptasensor assisted by immunomagnetic enrichment and enzyme signal amplification
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A simple, rapid and economic lateral flow immunochromatographic assay (LFICA) was designed for ultrasensitive detection of β-conglutin. Instead of antibodies and gold nanoparticles (AuNPs) used in conventional LFICA, a cognate aptamer duo, binding to β-conglutin and Fe3O4@Au core-shell nanoparticles, was applied in this study. An enzyme signal amplification strategy was used to enhance sensitivity. In addition, a new magnetic enrichment strategy was employed to further enhance sensitivity of the assay, slowing down movement of the capture probe (i.e., Fe3O4@Au nanostructures) using an external magnetic field. The novel LFICA assay can be completed within 20â¯min and achieved a detection limit of 8â¯fM, a thousand-times lower than similar assays without magnetic focusing. Overall, our results demonstrated the potential for the proposed LFICA sensor in rapid detection of β-conglutin without any special analytical expertise or instrumentations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 269, 15 December 2018, Pages 375-379
Journal: Food Chemistry - Volume 269, 15 December 2018, Pages 375-379
نویسندگان
Zhengzong Wu, Deyun He, Enbo Xu, Aiquan Jiao, Muhammad Farhan Jahangir Chughtai, Zhengyu Jin,