کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7585148 | 1492034 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The influence of food matrices on aptamer selection by SELEX (systematic evolution of ligands by exponential enrichment) targeting the norovirus P-Domain
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
This study investigates the enrichment of aptamers targeting the norovirus protruding domain in the presence of foods often associated with norovirus outbreaks. The goal is to explore if and how the presence of food alters in vitro selection of aptamers and target binding of the enriched oligonucleotides. Our study demonstrates that the introduction of food to SELEX (systematic evolution of ligands by exponential enrichment) is either detrimental to enrichment of oligonucleotides with target-specific binding, or facilitates enrichment of non-target-specific oligonucleotides. Moreover, a relationship between target binding of enriched oligonucleotides in presence of food and their selection condition was not observed. Our findings also suggest that a pathogen specific aptamer with application in food does not need to be selected in presence of the particular food, but may require properties beyond high affinity and selectivity to be applied for pathogen extraction and detection in undiluted food matrices.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 258, 30 August 2018, Pages 129-136
Journal: Food Chemistry - Volume 258, 30 August 2018, Pages 129-136
نویسندگان
Katja B. Schilling, Jeffrey DeGrasse, Jacquelina W. Woods,