کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7585543 | 1492039 | 2018 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Purification and identification of anti-inflammatory peptides from spent hen muscle proteins hydrolysate
ترجمه فارسی عنوان
خالص سازی و شناسایی پپتیدهای ضد التهابی از هیدرولیزات پروتئین های عضلانی نگهدارنده
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کلمات کلیدی
مرغ مصرفی پپتیدهای ضد التهابی، پاکسازی، شناسایی، طیف سنجی جرمی،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Spent hens have low market value and incur significant costs for disposal. This study explored the use of spent hens as a source of bioactive peptides. Spent hen hydrolysates prepared by Protease M or Protex 50FP exhibited interleukin (IL)-6 inhibitory activity in endotoxin-activated macrophage-like U937 cells (pâ¯<â¯.05). The potential peptides from Protex 50FP hydrolysate were further fractionated using a combination of ultrafiltration, solid-phase extraction and high-performance liquid chromatography; 17 novel peptides encoded in major muscle proteins were identified by mass spectrometry analysis, of which 7 were chemically synthesized and assayed for the IL-6 inhibitory activity. At a concentration of 100â¯Âµg/mL, peptide FLWGKSY induced a 79% reduction of IL-6 production in endotoxin-activated macrophage-like U937 cells, which is comparable to results reported from other food sources. Our results indicate that spent hens have potential to be a source of bioactive peptides for anti-inflammatory applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 253, 1 July 2018, Pages 101-107
Journal: Food Chemistry - Volume 253, 1 July 2018, Pages 101-107
نویسندگان
Wenlin Yu, Catherine J. Field, Jianping Wu,