کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7586006 | 1492043 | 2018 | 30 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An ultrasensitive aptasensor based on fluorescent resonant energy transfer and exonuclease-assisted target recycling for patulin detection
ترجمه فارسی عنوان
اپتاسنسور فوق حساس بر اساس انتقال انرژی رزونانس فلورسنت و بازیابی هدف مورد استفاده با اکسونوکراسی برای تشخیص پاتویلن
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Patulin (PAT) is a type of mycotoxin which can compromise both food quality and human health. Due to its harmful effects, strict monitoring of PAT contents in food systems is necessary. A novel kind of bioassay was proposed for determining PAT based on the fluorescent resonant energy transfer (FRET) strategy. The exonuclease-catalyzed target recycling strategy was employed to improve the sensitivity of the FRET system. The results showed that the linear range extends from 0.01â¯ng/mL to 100â¯ng/mL. Furthermore, the average recoveries ranged from 93.33% to 105.21%, confirming the reliability of this method. The total analysis time for our assay developed is about 50â¯min. Compared to traditional analytical methods, the developed assay is more stable and has a significantly lower detection of limit (0.003â¯ng/mL). We believe the approach developed in this study would be useful for high-throughput screening of PAT in food industry and government laboratory.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 249, 30 May 2018, Pages 136-142
Journal: Food Chemistry - Volume 249, 30 May 2018, Pages 136-142
نویسندگان
Zhengzong Wu, Enbo Xu, Zhengyu Jin, Joseph Irudayaraj,