کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7587685 | 1492079 | 2016 | 24 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development and validation of a HPLC method for determination of degree of polymerization of xylo-oligosaccharides
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A reliable reversed-phase HPLC method was developed for high resolution separation and high sensitivity determination of xylo-oligosaccharides (XOS) with degree of polymerization from 2 to 8. The method was carried out on a Kromasil C18 column using pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) and UV detection at 245Â nm. The effects of pH value of mobile phase, volume proportion of acetonitrile, concentration of ammonium acetate buffer and flow rate on the retention time and degree of separation of XOS derivatives were investigated. A satisfactory result was achieved in 25Â min with a mobile phase of 10Â mmol/L ammonium acetate buffer (pH5.5)-acetonitrile by a gradient elution at 0.8Â mL/min. In addition, this method was validated by liquid chromatography-tandem mass spectroscopy (LC-MS) analysis and several uncertain compounds were identified. The proposed HPLC method is suitable for the compositional analysis and quality control of XOS.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 213, 15 December 2016, Pages 654-659
Journal: Food Chemistry - Volume 213, 15 December 2016, Pages 654-659
نویسندگان
Jianghua Pu, Xia Zhao, Qingchi Wang, Yingdi Wang, Hui Zhou,