کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7588563 | 1492081 | 2016 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High resolution melting analysis as a new approach to discriminate gluten-containing cereals
ترجمه فارسی عنوان
تجزیه و تحلیل ذوب با وضوح بالا به عنوان یک رویکرد جدید برای تبعیض غلات حاوی گلوتن
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
With this work, it is intended to propose a novel approach based on high resolution melting (HRM) analysis to detect wheat and discriminate it from other gluten-containing cereals. The method consisted of a real-time PCR assay targeting the gene encoding for the germ agglutinin isolectin A protein (Tri a 18 allergen), using the fluorescent Evagreen dye combined with HRM analysis. The results enabled wheat differentiation from other phylogenetically related cereals, namely barley, rye and oat with high level of confidence. Additionally, a quantitative real-time PCR approach was proposed, allowing detecting and quantifying wheat down to 20Â mg/kg in rice flour and 20Â pg of wheat DNA (â¼1.1 DNA copies). Its application was successfully achieved in the analysis of processed foods to verify labelling compliance, being considered as a cost-effective tool for the specific detection of cereals in gluten-free foods.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 211, 15 November 2016, Pages 383-391
Journal: Food Chemistry - Volume 211, 15 November 2016, Pages 383-391
نویسندگان
Begoña MartÃn-Fernández, Joana Costa, Noemà de-los-Santos-Álvarez, Beatriz López-Ruiz, M. Beatriz P.P. Oliveira, Isabel Mafra,