کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7588563 1492081 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High resolution melting analysis as a new approach to discriminate gluten-containing cereals
ترجمه فارسی عنوان
تجزیه و تحلیل ذوب با وضوح بالا به عنوان یک رویکرد جدید برای تبعیض غلات حاوی گلوتن
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
With this work, it is intended to propose a novel approach based on high resolution melting (HRM) analysis to detect wheat and discriminate it from other gluten-containing cereals. The method consisted of a real-time PCR assay targeting the gene encoding for the germ agglutinin isolectin A protein (Tri a 18 allergen), using the fluorescent Evagreen dye combined with HRM analysis. The results enabled wheat differentiation from other phylogenetically related cereals, namely barley, rye and oat with high level of confidence. Additionally, a quantitative real-time PCR approach was proposed, allowing detecting and quantifying wheat down to 20 mg/kg in rice flour and 20 pg of wheat DNA (∼1.1 DNA copies). Its application was successfully achieved in the analysis of processed foods to verify labelling compliance, being considered as a cost-effective tool for the specific detection of cereals in gluten-free foods.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 211, 15 November 2016, Pages 383-391
نویسندگان
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