کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7589875 1492093 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
MALDI-TOF to compare polysaccharide profiles from commercial health supplements of different mushroom species
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
MALDI-TOF to compare polysaccharide profiles from commercial health supplements of different mushroom species
چکیده انگلیسی
In this work MALDI-TOF mass spectroscopy was investigated to characterise the β-glucan profiles of several commercial health supplements, without any derivatisation or purification pre-treatment. The effect of two solvents (water and dimethyl sulfoxide) and two MALDI matrices (2,5-dihydroxybenzoic acid and 2′,4′,6′-trihydroxyacetophenone) was first evaluated on dextran standards. MALDI-TOF was found as a useful and quick technique to obtain structural information of diverse food supplements based on mushroom extracts. The MALDI polysaccharide profiles of 5 supplements from different mushroom species were qualitatively similar showing [Glucan + Na]+ cations with a peak-to-peak mass difference of 16 Da consistent with the repeating unit of the β-(1 → 3)-glucan. The profiles strongly depended on the sample solvent used, with m/z values around 5000-8000 for water and 2000 for dimethyl sulfoxide; differences between samples were revealed in the molecular weight of the aqueous preparation, with the highest values for Maitake and Cordyceps species.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 199, 15 May 2016, Pages 597-604
نویسندگان
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