کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7591694 | 1492104 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rapid method for the determination of 14 isoflavones in food using UHPLC coupled to photo diode array detection
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Rapid method for the determination of 14 isoflavones in food using UHPLC coupled to photo diode array detection Rapid method for the determination of 14 isoflavones in food using UHPLC coupled to photo diode array detection](/preview/png/7591694.png)
چکیده انگلیسی
A rapid method for the determination of 14 types of isoflavones in food using ultra-high performance liquid chromatography (UHPLC) was validated in terms of precision, accuracy, sensitivity and linearity. The UHPLC separation was performed on a reverse-phase C18 column (particle size 2 μm, i.d. 2 mm, length 100 mm) using a photo diode array detector that was fixed to 260 nm. The limits of detection and quantification of the UHPLC analyses ranged from 0.03 to 0.33 mg kgâ1. The intra-day and inter-day precision of the individual isoflavones were less than 11.77% and calibration curves exhibited good linearity (r2 = 0.99) within the tested ranges. These results suggest that the rapid method used in this study could be available to determine of 14 types of isoflavones in a variety of food such as soy bean, black bean, red bean and soybean paste.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 187, 15 November 2015, Pages 391-397
Journal: Food Chemistry - Volume 187, 15 November 2015, Pages 391-397
نویسندگان
You-Shin Shim, Won-Jin Yoon, Jin-Bong Hwang, Hyun-Jin Park, Dongwon Seo, Jaeho Ha,