کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7593003 | 1492115 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differentiation between fresh and frozen-thawed sea bass (Dicentrarchus labrax) fillets using two-dimensional gel electrophoresis
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کلمات کلیدی
Dithiothreitol (PubChem CID: 19001)Perchloric acid (PubChem CID: 24247)Sea bass (Dicentrarchus labrax)iodoacetamide (PubChem CID: 3727)Biogenic amines - آمین های زیستی یا آمین بیوژنیکEthanol (PubChem CID: 702) - اتانول (PubChem CID: 702)Acetonitrile (PubChem CID: 6342) - استونیتریل (PubChem CID: 6342)Formic acid (PubChem CID: 284) - اسید فرمیک (PubChem CID: 284)2D-electrophoresis - الکتروفورز 2DUrea (PubChem CID: 1176) - اوره (PubChem CID: 1176)Fresh - تازه
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
This study aimed to identify a protein marker that can differentiate between fresh skinless and frozen-thawed sea bass (Dicentrarchus labrax) fillets using the two-dimensional polyacrylamide gel electrophoresis (2-DE) technique. Distinct gel patterns, due to proteins with low molecular weight and low isoelectric points, distinguished fresh fillets from frozen-thawed ones. Frozen-thawed fillets showed two specific protein spots as early as the first day of the study. However, these spots were not observed in fresh fillets until at least 13 days of storage between 0 and 4 °C, fillets were judged, beyond this period, fish were unfit for human consumption as revealed by complementary studies on fish spoilage indicators namely total volatile basic nitrogen and biogenic amines. Mass spectrometry identified the specific proteins as parvalbumin isoforms. Parvalbumins may thus be useful markers of differentiation between fresh and frozen-thawed sea bass fillets.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 176, 1 June 2015, Pages 294-301
Journal: Food Chemistry - Volume 176, 1 June 2015, Pages 294-301
نویسندگان
Pierrette Ethuin, Sylvain Marlard, Mylène Delosière, Christine Carapito, François Delalande, Alain Van Dorsselaer, Alexandre Dehaut, Valérie Lencel, Guillaume Duflos, Thierry Grard,