کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7594160 | 1492117 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of alditols by capillary electrophoresis with indirect laser-induced fluorescence detection
ترجمه فارسی عنوان
تعیین الیدول توسط الکتروفورز مویرگی با تشخیص فلورسانس ناشی از لیزر غیر مستقیم
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کلمات کلیدی
آلدیتول، الکتروفورز مویرگی، فلورسانس ناشی از لیزر،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A novel method was developed based on capillary electrophoresis with indirect laser-induced fluorescence detection for the determination of alditols including xylitol, sorbitol, arabinitol and mannitol. The baseline separation was achieved with a capillary with 30 μm inner diameter and running buffer (pH 9.6) composed of 40 mM sodium tetraborate, 5% (v/v) methanol, 10â5 M sodium fluorescein as background fluorescence. Calibration curves (0.2-2.0 mg mLâ1) showed good linear correlations (r > 0.999). The detection limits of the alditols were in the range of 19.0-24.4 μg mLâ1. The intra-day and inter-day RSDs for migration times and peak areas were less than 1.6% and 4.3%, respectively. The average recoveries were in the range of 99.6-105.5%. The developed method was successfully applied to determination of alditols in different real samples, and the results were compared with those of capillary electrophoresis with indirect ultraviolet detection method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 174, 1 May 2015, Pages 233-239
Journal: Food Chemistry - Volume 174, 1 May 2015, Pages 233-239
نویسندگان
Yao Xiao, Yunfang Li, Jinjin Ying, Yu Tian, Yuxiu Xiao, Zhinan Mei,