کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7596760 1492127 2014 26 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Optimisation extraction of chondroitin sulfate from fish bone by high intensity pulsed electric fields
ترجمه فارسی عنوان
استخراج بهینه سازی کاندرویتین سولفات از استخوان ماهی با میدان های الکتریکی پالس با شدت بالا
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
High intensity pulsed electric fields (PEF) was used to extract chondroitin sulphate (CS) from fish bone. Results show that PEF extraction speed is much faster, and the content of CS is much higher compared with traditional methods. Variation of PEF parameters and the content of CS were determined by single factor experiments. The processing conditions were optimised by quadratic general rotary unitised design experiments. The maximum yield of 6.92 g/L was achieved under the following conditions: material-liquid ratio of 1:15 g/mL, electric field intensity of 16.88 kV/cm, pulse number of 9, and NaOH concentration of 3.24%. The purity of CS was analysed by agarose gel electrophoresis. CS purity was high, and the extract did not contain any other glycosaminoglycans. PEF can be widely used to extract CS with non-thermal performance, high speed, and low pollution.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 164, 1 December 2014, Pages 205-210
نویسندگان
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