کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7598239 | 1492133 | 2014 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A rapid immunomagnetic beads-based immunoassay for the detection of β-casein in bovine milk
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A rapid immunomagnetic beads-based immunoassay for the detection of β-casein in bovine milk A rapid immunomagnetic beads-based immunoassay for the detection of β-casein in bovine milk](/preview/png/7598239.png)
چکیده انگلیسی
An immunomagnetic beads-based enzyme-linked immunosorbent assay (IMBs-ELISA) was developed for the detection of β-casein in bovine milk. Immunomagnetic beads (IMBs) were employed as the solid phase. The anti-β-casein monoclonal antibody (McAb) bound to IMBs was used as capture probe and an anti-β-casein polyclonal antibody (PcAb), labelled with horseradish peroxidase (HRP), was employed as detector probe. Three reaction and two washing steps were needed. Each reaction needed 10 min or less, which significantly shortened detection compared with classic sandwich ELISA. β-Casein in bovine milk was detected across a linear range (2-128 μg mLâ1). Application results were in accordance with the Kjejdahl method, which suggests the IMBs-ELISA is rapid and reliable for the detection of β-casein in bovine milk.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 158, 1 September 2014, Pages 445-448
Journal: Food Chemistry - Volume 158, 1 September 2014, Pages 445-448
نویسندگان
F. Song, Y. Zhou, Y.S. Li, X.M. Meng, X.Y. Meng, J.Q. Liu, S.Y. Lu, H.L. Ren, P. Hu, Z.S. Liu, Y.Y. Zhang, J.H. Zhang,