کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7599179 1492141 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fluorescent immunosorbent assay for the detection of alpha lactalbumin in dairy products with monoclonal antibody bioconjugated with CdSe/ZnS quantum dots
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Fluorescent immunosorbent assay for the detection of alpha lactalbumin in dairy products with monoclonal antibody bioconjugated with CdSe/ZnS quantum dots
چکیده انگلیسی
In this work, a new method termed competitive fluorescence-linked immunosorbent assay (FLISA) was developed for specifically quantification of bovine α-lactalbumin (α-La) in dairy products. The monoclonal antibodies (mAbs) against α-La were produced through hybridoma technology, and the mAbs were covalently conjugated with the CdSe/ZnS quantum dots (QDs) using the crossing-linking reagents. Moreover, a competitive FLISA based on QD-mAb conjugates was established to detect α-La in dairy products. It was shown that there was a good linear relationship between inhibition efficiency, and logarithm of α-La concentration after the detection parameters were optimised in which the concentration of α-La varied from 0.1 to 1000 ng/mL. The value of IC50 was 0.03 μg/mL, and the FLISA method exhibited high sensitivity with the LOD at 0.1 ng/mL. The developed FLISA has been successfully applied to determine α-La in commercial dairy products, providing more sensitive analysis compared with the ELISA method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 150, 1 May 2014, Pages 73-79
نویسندگان
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