کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7600038 1492144 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid tea catechins and caffeine determination by HPLC using microwave-assisted extraction and silica monolithic column
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Rapid tea catechins and caffeine determination by HPLC using microwave-assisted extraction and silica monolithic column
چکیده انگلیسی
A rapid reversed-phase high performance liquid chromatographic method using a monolithic column for the determination of eight catechin monomers and caffeine was developed. Using a mobile phase of water:acetonitrile:methanol (83:6:11) at a flow rate of 1.4 mL min−1, the catechins and caffeine were isocratically separated in about 7 min. The limits of detection and quantification were in the range of 0.11-0.29 and 0.33-0.87 mg L−1, respectively. Satisfactory recoveries were obtained (94.2-105.2 ± 1.8%) for all samples when spiked at three concentrations (5, 40 and 70 mg L−1). In combination with microwave-assisted extraction (MAE), the method was applied to the determination of the catechins and caffeine in eleven tea samples (6 green, 3 black and 2 oolong teas). Relatively high levels of caffeine were found in black tea, but higher levels of the catechins, especially epigallocatechin gallate (EGCG) were found in green teas.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Chemistry - Volume 147, 15 March 2014, Pages 262-268
نویسندگان
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