A potential practical approach to reduce Ara h 6 allergenicity by gamma irradiation

► Irradiation induced structural alteration of peanut allergen Ara h 6 was investigated. ► Irradiation induced antigenic changes of Ara h 6 and peanut protein extract was investigated. ► Irradiation has induced significant changes in the secondary and tertiary structure of Ara h 6. ► The antigenicity of purified Ara h 6 and peanut protein extract were reduced upon irradiation. ► A good correlation between the loss in α-helix and IgG binding to Ara h 6 was observed.