The devolopment of duplex real-time PCR based on SYBR Green florescence for rapid ıdentification of ruminant and poultry origins in foodstuff

► The development of duplex real-time PCR based on SYBR Green for rapid identification. ► Design of one of primers to amplify the ruminant mitochondrial 16S rRNA-tRNA. ► This assay is cheaper than assay with probes to detect a group of mixed meats. ► First time, ruminant and poultry have been detected in simultaneous reaction by SYBR Green. ► SDRT-PCR has, saving time, cost, and material.