Structural characterization of both the non-proteolytic and proteolytic activation pathways of coagulation Factor XIII studied by hydrogen-deuterium exchange mass spectrometry

▶ FXIII can be activated both via a proteolytic pathway and a non-proteolytic pathway. ▶ Weakened dimer interaction is observed in rFXIIIa′, rFXIIIa* and rFXIIIå. ▶ Extensive conformational changes occur upon full activation to either rFXIIIa* or rFXIIIå. ▶ Both activation pathways of rFXIII produce similar activated conformations.