The development and validation of a LC-MS/MS method for the quantitation of metformin, rifampicin and isoniazid in rat plasma using HILIC chromatography

- The first LC-MS/MS method for the simultaneous quantitation of MET, RIF and INH.
- The method requires 20 μl of plasma and a short sample run time of 4 min.
- HILIC chromatography was used to separate small polar and hydrophobic compounds.
- The method was applied to a bioavailability study in rats.
- Co-administration of RIF and INH increased the bioavailability (AUC) of MET.