کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7614818 | 1493977 | 2018 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Dry Blood Spot sample collection for post-exposure monitoring of chemical warfare agents - In vivo determination of phosphonic acids using LC-MS/MS
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
Phosphonic acids are the direct and immediate metabolites of organophosphorus chemical warfare agents (OP-CWAs). Accordingly, their detection serves for evaluating exposure to OP-CWAs in a terror or war scenario. After exposure, phosphonic acids are present in the blood; however, blood drawing must be carried out by medical personnel, hence the number of samples that can be drawn in a mass-casualty event is limited. Herein, we describe a new approach developed for the determination of phosphonic acids in blood using Dry Blood Spots (DBSs) on a filter paper. The method is based on a simple sample preparation protocol, followed by LC-MS-MS targeted (MRM) analysis. The detection limits of Soman (GD), Cyclosarin (GF) and VX metabolites in whole blood were as low as 1â¯ng/ml, while the detection limits were 0.3â¯ng/ml for the GF metabolite and 0.5â¯ng/ml for the Sarin (GB) metabolite. Good recoveries were obtained in the range of 1-100â¯ng/ml for GB and GD metabolites, and 3-100â¯ng/ml for GF, VX and RVX metabolites, with a linear response (R2â¯=â¯0.99). The method has proven to be reliable even with DBS samples stored up to 35â¯days at room temperature before analysis. This method was implemented in a 24â¯h time-course determination of the Sarin metabolite in an in - vivo experiment, after rat exposure to 1 LD50 of Sarin. This technique is simple, rapid, sensitive, robust, long lasting and compatible with field collection and storage; hence, it can serve for large-scale sampling and reliable monitoring of potential OP-CWAs casualties. Since DBS sampling is amenable to nonprofessionals, including self-sampling, this technique is highly suitable for mass-casualty incidents.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volumes 1093â1094, 1 September 2018, Pages 60-65
Journal: Journal of Chromatography B - Volumes 1093â1094, 1 September 2018, Pages 60-65
نویسندگان
Lilach Yishai Aviram, Miriam Magen, Shira Chapman, Adi Neufeld Cohen, Shlomi Lazar, Shai Dagan,