کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7614839 | 1493976 | 2018 | 17 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Simultaneous determination of the novel anti-tumor candidate drug MDH-7 and 5-fluorouracil in rat plasma by LC-MS/MS: Application to pharmacokinetic interactions
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
MDH-7 (2,3,9-tri-O-acetyl-5,6-dideoxy-1,10-di-[N4â²-pentoxycarbonyl-5â²-fluoro cytosine]-4-ulose 1,4: 7,10-difuranose-4,8-pyranose) is a novel anti-tumor drug candidate. To study the pharmacokinetic interaction between MDH-7 and 5-fluorouracil (5-FU), a sensitive and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed to simultaneously determine the concentrations of MDH-7 and 5-fluorouracil (5-FU) in rat plasma. Plasma samples were prepared by simple liquid-liquid extraction with ethyl acetate. Chromatographic separation was performed on a Waters XBridge⢠C18 column (5â¯Î¼m, 2.1â¯mmâ¯Ãâ¯150â¯mm) with the mobile phase of methanol and H2O (80:20, v/v). The ESI positive and negative ion switch was operated in the multiple reactions monitoring (MRM) mode. The calibration curves showed good linearity (r2â¯>â¯0.99) over the ranges of 50-8000â¯ng/mL for MDH-7 and 10-2000â¯ng/mL for 5-FU, respectively. The lower limit of quantitations (LLOQs) was 50â¯ng/mL (MDH-7) and 10â¯ng/mL (5-FU) with relative standard deviation (RSD)â¯<â¯13.0%. The proposed method was successfully applied to simultaneous assessment of pharmacokinetic drug-drug interaction between MDH-7 and 5-FU in rats.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1095, 15 September 2018, Pages 235-240
Journal: Journal of Chromatography B - Volume 1095, 15 September 2018, Pages 235-240
نویسندگان
Juan Li, Yu Chen, Piao Zhou, Qiqi Fan, Qingli Wang, Xiaorang Chen, Yingqian Su, Yanyan Diao, Meili Guo, Mengchen An, Hongmin Liu,