کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7614865 | 1493978 | 2018 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A targeted strategy to identify untargeted metabolites from in vitro to in vivo: Rapid and sensitive metabolites profiling of licorice in rats using ultra-high performance liquid chromatography coupled with triple quadrupole-linear ion trap mass spectrome
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کلمات کلیدی
XICUHPLCMRMQTrapqTOFHRMSEPILC–MS/MS - LC-MS / MSUHPLC-MS/MS - UHPLC-MS / MSenhanced product ion scan - افزایش اسکن یون محصولQuadrupole time of flight - زمان پرواز چهار روزهLicorice - شیرین بیانtraditional Chinese medicine - طب سنتی چینیHigh resolution mass spectrometry - طیف سنجی جرمی با وضوح بالاMetabolites - متابولیت هاmultiple reaction monitoring - نظارت چندگانه چندگانهhigh performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاUltra-high performance liquid chromatography - کروماتوگرافی مایع با کارایی فوق العاده بالاliquid chromatography coupled with tandem mass spectrometry - کروماتوگرافی مایع همراه با طیف سنجی جرمی دو طرفهHPLC - کروماتوگرافی مایعی کاراextracted ion chromatogram - کروماتوگرافی یون استخراج شده
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
It is challenging to conduct in vivo metabolic study for traditional Chinese medicines (TCMs) because of complex components, unpredictable metabolic pathways and low metabolite concentrations. Herein, we proposed a sensitive strategy to characterize TCM metabolites in vivo at an orally clinical dose using ultra-high performance liquid chromatography-triple quadrupole-linear ion trap mass spectrometry (UHPLC-QTRAP-MS). Firstly, the metabolism of individual compounds in rat liver microsomes was studied to obtain the metabolic pathways and fragmentation patterns. The untargeted metabolites in vitro were detected by multiple ion monitoring-enhanced product ion (EPI) and neutral loss-EPI scans. Subsequently, a sensitive multiple reaction monitoring-EPI method was developed according to the in vitro results and predicted metabolites to profile the in vivo metabolites. Licorice as a model herb was used to evaluate and validate our strategy. A clinical dose of licorice water extract was orally administered to rats, then a total of 45 metabolites in urine, 21 metabolites in feces and 35 metabolites in plasma were detected. Among them, 18 minor metabolites have not been reported previously and 6 minor metabolites were first detected in vivo. Several isomeric metabolites were well separated and differentiated in our strategy. These results suggested that this new strategy could be widely used for the detection and characterization of in vivo metabolites of TCMs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1092, 15 August 2018, Pages 40-50
Journal: Journal of Chromatography B - Volume 1092, 15 August 2018, Pages 40-50
نویسندگان
Meilin Huang, Zhongzhe Cheng, Lu Wang, Yulin Feng, Jiangeng Huang, Zhifeng Du, Hongliang Jiang,