کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7614933 | 1493978 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of aflatoxin and zearalenone analogs in edible and medicinal herbs using a group-specific immunoaffinity column coupled to ultra-high-performance liquid chromatography with tandem mass spectrometry
ترجمه فارسی عنوان
تعیین آنالوگ آفلاتوکسین و زئیرالنون در گیاهان دارویی و گیاهی با استفاده از یک ستون ایمونوفیبین اختصاصی گروهی به همراه کروماتوگرافی مایع با کیفیت بالا با اسپکترومتراسیون دو طرفه
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Six aflatoxins (AFs; AF B1, B2, G1, G2, M1 and M2) and six zearalenone (ZEN) analogs (ZEN, zearalanone, α-zeralanol, β-zeralanol, α-zearalenol, and β-zearalenol) were simultaneously extracted from edible and medicinal herbs using a group-specific immunoaffinity column (IAC) and then identified by ultra-high-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). The IAC was prepared by coupling N-hydroxysuccinimide-activated Sepharose 4B Fast Flow gel with two group-specific monoclonal antibodies. The column capacities to six AFs and six ZEN analogs ranged from 100.2â¯ng to 167.1â¯ng and from 59.5â¯ng to 244.4â¯ng, respectively. The IAC-UPLC-MS/MS method was developed and validated with three different matrices (Chinese yam [Dioscorea polystachya], Platycodon grandiflorum and coix seed [Semen Coicis]). Recoveries of twelve analytes from edible and medicinal herbs were in the range of 64.7%-112.1%, with relative standard deviations below 13.7%. The limits of quantification were in the range from 0.08â¯Î¼gâ¯kgâ1 to 0.2â¯Î¼gâ¯kgâ1. The method was proven to be sensitive and accurate, and suitable for the determination of real samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1092, 15 August 2018, Pages 228-236
Journal: Journal of Chromatography B - Volume 1092, 15 August 2018, Pages 228-236
نویسندگان
Shujuan Sun, Kai Yao, Sijun Zhao, Pimiao Zheng, Sihan Wang, Yuyang Zeng, Demei Liang, Yuebin Ke, Haiyang Jiang,