کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7615041 | 1493982 | 2018 | 29 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Simultaneous determination of cyclosporine and tacrolimus in human whole blood by ultra-high performance liquid chromatography tandem mass spectrometry and comparison with a chemiluminescence microparticle immunoassay
ترجمه فارسی عنوان
تعیین همزمان سیکلوسپورین و تاکرولیموس در کل خون انسان با استفاده از اسپکترومتر جرمی کروماتوگرافی مایع با عملکرد بالا و مقایسه آن با یک آزمایش ایمونوکلئیک میکرولومینسانس
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Overestimation of immunoassays for cyclosporine (CsA) and tacrolimus (TAC) analysis in human whole blood is a problem. The liquid chromatography tandem mass spectrometry is recommended as a golden method for CsA and TAC analysis. The aim of the study is to develop and validate an ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method for simultaneous determination of CsA and TAC in human whole blood and evaluate its agreement with a chemiluminescence microparticle immunoassay (CMIA). The UHPLC-MS/MS method for simultaneous determination of CsA and TAC in human whole blood was developed and validated according to the guidelines. A total of 177 CsA and 220 TAC samples were determined by UHPLC-MS/MS and CMIA, and the agreement of the two methods was evaluated by Bland-Altman plot. The calibration range of UHPLC-MS/MS method was 5 to 2000â¯ng/mL for CsA and 0.2 to 80â¯ng/mL for TAC. The inaccuracy and imprecision were â13.33% to 11.80% and <11.74% for CsA and â8.94% to 6.53% and <10.84% for TAC, respectively. Evaluated by Bland-Altman plot, the mean overestimation of CMIA compared to UHPLC-MS/MS was 53.7% for CsA and 48.1% for TAC.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volumes 1087â1088, 15 June 2018, Pages 36-42
Journal: Journal of Chromatography B - Volumes 1087â1088, 15 June 2018, Pages 36-42
نویسندگان
Shenghui Mei, Jiaqing Wang, Di Chen, Leting Zhu, Ming Zhao, Xiaoxin Tian, Xin Hu, Zhigang Zhao,