کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7615088 | 1493984 | 2018 | 26 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A LC/MS/MS method for determination of tenofovir in human plasma and its application to toxicity monitoring
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A LC/MS/MS method for determination of tenofovir in human plasma and its application to toxicity monitoring A LC/MS/MS method for determination of tenofovir in human plasma and its application to toxicity monitoring](/preview/png/7615088.png)
چکیده انگلیسی
Tenofovir disoproxil fumarate is a pro-drug of the active metabolite tenofovir widely used against the HIV1, HIV2, and Hepatitis B virus. Several studies have been conducted and found kidney injury associated with tenofovir exposure. High tenofovir plasma concentration correlated with kidney injury in tenofovir-exposed patients. The present study developed and validated a simple and cost-effective LC/MS/MS method to determine tenofovir level in human plasma. A small plasma volume of 80â¯Î¼l is utilized for the sample preparation. The samples were separated by Luna C18 (100â¯mmâ¯Ãâ¯2.0â¯mm, 3â¯Î¼m) using gradient elution with a mobile phase consisting of water (containing 0.1% formic acid) and acetonitrile (90:10, v/v). The detection was achieved through multiple reaction monitoring using positive ionization mode on the triple quadrupole mass spectrometer with a run time of 10â¯min. The monitoring transitions were set at m/z 288.0â¯ââ¯176.1 and 136.1 for tenofovir and m/z 226.1â¯ââ¯152.0 for acyclovir (as the internal standard). This standard curve was linear from 10 to 640â¯ng/ml, with the lower limit of quantification of 10â¯ng/ml. The inter- and intra-day precision results were less than 12.3% and their accuracies were within the acceptable range of 84.9-113.1%. The validated method was successfully applied to the study of tenofovir induced kidney injury in HIV-1 infected patients taking 300â¯mg once daily for more than 4â¯weeks.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1085, 15 May 2018, Pages 89-95
Journal: Journal of Chromatography B - Volume 1085, 15 May 2018, Pages 89-95
نویسندگان
Narit Wiriyakosol, Apichaya Puangpetch, Weerawut Manosuthi, Sasinapha Tomongkon, Chonlaphat Sukasem, Darawan Pinthong,