کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7615389 | 1493991 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
LC-MS/MS bioassay of four proton pump inhibitors
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: LC-MS/MS bioassay of four proton pump inhibitors LC-MS/MS bioassay of four proton pump inhibitors](/preview/png/7615389.png)
چکیده انگلیسی
A new validated bio-analytical LC-MS/MS method was developed for the simultaneous extraction and determination of four proton pump inhibitors: esomeprazole, lansoprazole, pantoprazole and rabeprazole in human plasma using escitalopram as an internal standard. The proteins in plasma samples were precipitated using acetonitrile for the extraction of analytes which is a simple economic method. The separation was accomplished using a mobile phase composed of 10â¯mM ammonium formate: acetonitrile: methanol (20:40:40% v/v) at a flow rate of 0.8â¯mL/min in isocratic mode on a reversed phase C18 INERTSIL ODS-3 (5â¯Î¼m, 150â¯Ãâ¯4.6â¯mm) and column temperature of 40â¯Â°C. Positive mode electrospray ionization source was used prior to multiple reaction monitoring (MRM) detection using parent and daughter ions: m/z 346.2â¯ââ¯198.1 for esomeprazole, m/z 370.1â¯ââ¯252 for lansoprazole, m/z 384.2â¯ââ¯200.2 for pantoprazole, m/z 360.1â¯ââ¯242.1 for rabeprazole and m/z 325.2â¯ââ¯109 for escitalopram. The calibration curves were constructed, and the method was linear in the range of 20-5000â¯ng/mL applying weighted (1/X2) linear regression coefficient for all drugs. The method was fully validated following US-FDA and EMA guidelines.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1076, 15 February 2018, Pages 61-69
Journal: Journal of Chromatography B - Volume 1076, 15 February 2018, Pages 61-69
نویسندگان
Ehab F. Elkady, Marwa A. Fouad, Bushra M. Jaadan,