کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7615947 | 1494028 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Simple and validated UHPLC-MS/MS analysis of nimodipine in plasma and cerebrospinal fluid of patients with subarachnoid haemorrhage
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کلمات کلیدی
LLOQSRMLLESAHUHPLCLCSlacosamideaCSFNimodipinenim - آنI.S. - است.internal standard - استاندارد داخلیLiquid-liquid extraction - استخراج مایع _ مایعlower limit of quantification - حد پایین کمیت سنجیAneurysmal subarachnoid haemorrhage - خونریزی زیراراخنوئیدی غیرطبیعیSubarachnoid haemorrhage - خونریزی سوباراکونوئیدIntravenous - داخل وریدیLLOD - روزPlasma concentration - غلظت پلاسماCSF - مایع مغزی نخاعیCerebrospinal fluid - مایع مغزی نخاعیartificial cerebrospinal fluid - مایع مغزی نخاعی مصنوعیlower limit of detection - محدودیت پایین تشخیصselected reaction monitoring - نظارت بر واکنش انتخاب شدهquality control - کنترل کیفیت
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We present a simple, fast and validated method for the determination of nimodipine in plasma and cerebrospinal fluid (CSF) of patients with subarachnoid haemorrhage using ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Plasma or CSF 250 μL aliquots were pretreated with acetonitrile spiked with lacosamide as internal standard. The chromatographic separation was performed on a Fusion (3 μm) 50 Ã 2.0 mm I.D. column with gradient elution of 0.1% (v/v) formic acid in water and 0.1% (v/v) formic acid in acetonitrile at a flow rate of 0.35 mL/min. The MS/MS ion transitions were 419.1 â 343 for nimodipine and 251.1 â 91 for the internal standard. The linearity was determined from 2.0 to 40.0 ng/mL in plasma and 40.0-800.0 pg/mL in CSF. The lower limit of quantitation (LLOQ) of nimodipine was 0.4 ng/mL in plasma and 40 pg/mL in CSF. The mean recovery for nimodipine was â¥75% in plasma and â¥90% in CSF at all three considered concentrations. Intra- and interassay precision and accuracy were â¤15% at all quality control concentrations in plasma and CSF. The method was applied to measure plasma and CSF concentrations of nimodipine in a series of patients with subarachnoid haemorrhage treated with intravenous nimodipine. The present procedure, omitting time-consuming liquid-liquid extraction and drying steps, is faster, simpler and cheaper than published LC-MS/MS analytical methods for nimodipine in plasma and the first validated one for nimodipine in CSF.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1028, 15 August 2016, Pages 94-99
Journal: Journal of Chromatography B - Volume 1028, 15 August 2016, Pages 94-99
نویسندگان
Susan Mohamed, Roberto Riva, Manuela Contin,