کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7616665 | 1494045 | 2015 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Solvent modulation strategy for superior antibody monomer/aggregate separation in cation exchange chromatography
ترجمه فارسی عنوان
استراتژی مدولاسیون حلال برای جداسازی مونومر / جامد آنتی بادی برتر در کروماتوگرافی تبادل کاتیون
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
This new solvent modulation strategy was tested with three different mAbs on two different CEX resins in the presence of PEG in combination with various solubility enhancers. In order to assess the general applicability of this method, mAbs were selected that show major differences with respect to their sensitivity to PEG-induced precipitation and monomer/aggregate resolution performance that is achieved by CEX under standard conditions. For all three mAbs precipitation could be prevented without elimination of the positive PEG-effect. The addition of solubility enhancers gives access to improved separation at elevated PEG concentrations and high protein loadings without running into precipitation issues. Our data indicate that this method is generically applicable and leads to a superior antibody monomer/aggregate separation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1006, 1 December 2015, Pages 37-46
Journal: Journal of Chromatography B - Volume 1006, 1 December 2015, Pages 37-46
نویسندگان
Simon Kluters, Christian Frech, Thomas von Hirschheydt, Andreas Schaubmar, Sebastian Neumann,